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| 规格 | 价格 | 期货 | 数量 |
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Immunoprecipitating GAPDH in Hela whole cell lysate with mouse anti GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody at 1:5000 dilution, and followed by the AbFlour 800 labeled anti-Mouse IgG antibody at 1:5000 dilution. Lane 1 (Input): 20ug Hela whole cell lysate. Lane 2 (-):Mouse monoclonal IgG1 Isotype Control instead of GAPDH antibody in Hela whole cell lysate. Lane 3 (+): 6ug GAPDH antibody IP in 1000ug Hela whole cell lysate |
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Mouse IgG for IP was diluted at 1:3000 to detect non-reduced or reduced different forms of mouse IgG, 1ug/lane |
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